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Oligo Modifications List | Oligo Modifications Reference Category
Modification : Biotin dT
Reference Catalog Number 26-6424
Category Affinity Ligands
Modification Code Bio-dT
5 Prime Y
3 Prime Y
Internal Y
Molecular Weight (mw) 684.7
Extinction Coeficient (ec) 8.4
Technical Info (pdf) PS26-6424.pdf
Catalog NoScalePrice
26-6424-0550 nmol$325.00
26-6424-02200 nmol$325.00
26-6424-011 umol$422.50
26-6424-032 umol$633.75
26-6424-1010 umol$3,380.00
26-6424-1515 umol$4,225.00

Discounts are available for Biotin dT!
Modification* Discount Price Structure
1 site/order List price
2 sites/order 10% discount
3 sites/order 20% discount
4 sites/order 30% discount
5-9 sites/order 50% discount
10+ sites/order 60% discount
*Exceptions apply

Related Modifications
Biotin TEG
Biotin-SS-NHS-Sulfo
Biotin
BiotinTEG Azide
Biotin NHS
Biotin Multi

Biotin-dT is a deoxythymidine nucleotide base attached to biotin through a long-chain alkyl spacer arm. Biotin-dT is typically used to label an oligonucleotide with biotin at an internal base position, though it can also be used for 5’- and 3’-end labeling as well. Internal biotin-labeling is necessary when an oligo needs to be captured or immobilized, but both ends are unavailable. A good example of this is the use of internal biotin-labeling to facilitate the immobilization of a molecular beacon on a glass slide (1). The internal labeling strategy can also be used to label a hybridization detection probe with several biotins to enhance detection of a target sequence with anti-biotin antibodies attached to colored particles (2), fluorescent dyes, or enzymes.

References
1. Fang, X., Liu, X., Schuster, S., Tan, W. Designing a novel molecular beacon for surface immobilized DNA hybridization studies. J. Am. Chem. Soc. (1999), 121: 2921-2922.
2. Dineva, M.A., Candotti, D., Fletcher-Brown, F., Allain, J-P., Lee, H. Simultaneous Visual Detection of Multiple Viral Amplicons by Dipstick Assay.J. Clin. Microbiol. (2005), 43: 4015-4021.
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