Home | Products | Oligo Technology | Fluorescent Probes | Modifications | Oligo Design Tools | Online Order

Oligo Modifications List | Oligo Modifications Reference Category
Modification : PC Spacer (photocleavable)
Reference Catalog Number 26-6889
Category Photo Cleavable
Modification Code SpPC
5 Prime Y
3 Prime Y
Internal Y
Molecular Weight (mw) 344.26
Technical Info (pdf) PS26-6889.pdf
Catalog NoScalePrice
26-6889-0550 nmol$170.00
26-6889-02200 nmol$170.00
26-6889-011 umol$250.00
26-6889-032 umol$415.00
26-6889-1010 umol$1,680.00
26-6889-1515 umol$2,100.00

Discounts are available for PC Spacer (photocleavable)!
Modification* Discount Price Structure
1 site/order List price
2 sites/order 10% discount
3 sites/order 20% discount
4 sites/order 30% discount
5-9 sites/order 50% discount
10+ sites/order 60% discount
*Exceptions apply

Related Modifications
PC Biotin (photocleavable)
PC Amino C6 (Photocleavable)
PC Linker (photocleavable)
rSpacer
Spacer 18
Spacer 9
Spacer C3
Spacer C12

PC Spacer (photocleavable) is a non-nucleosidic molety that can be used as an intermediary to attach any available phosphoramidite modification at either end of an oligonucleotide through a UV photo-cleavable C3 spacer, as well as insert such a spacer internally. An example is the use of PC Spacer to incorporate a photo-cleavable 6-FAM tag onto the 5’-end of oligonucleotides immobilized on glass slides. These fluorescently-labeled oligo arrays were then UV irradiated in order to test the efficacy of photo-cleavage in removing the 6-FAM tag from these oligos, as part of developing sequencing-by-synthesis applications (1).

Cleavage Protocol


Cleavage occurs by irradiation with near-UV light (300-350 nm, complete cleavage occurs within 5 minutes. Try using a Black Ray XX-15 UV lamp (Ultraviolet Products Inc., San Gabriel, CA) at a distance of 15 cm (emission peak 365 nm, 300 nm cut-off, 1.1 mW intensity at~31 cm).

References


1. Olejnik, J., Krzymanska-Olejnik, E., Rothschild, K.J. Photocleavable aminotag phosphoramidites for 5’-termini DNA/RNA labeling. Nucleic Acids Res. (1998), 26: 3572-3576.
2. Olejnik, J., Ludemann, H-C., Olejnik, E.K, Berkenkamp, S., Hillenkamp, F., Rothschild, K.J. Photocleavable peptide-DNA conjugates: synthesis and applications to DNA analysis using MALDI-MS. Nucleic Acids Res. (1999), 27: 4626-4631.
3. Tang, X., Su, M., Yu, LiLi, Lv, C., Wang, J., Li, Z. Photomodulating RNA cleavage using photolabile circular antisense oligodeoxynucleotides.Nucleic Acids Res. (2002), 38: 3848-3855.
Email
Question

Enter the letters you see above for verification:
Order Status | Customer Service | Site Map | Request Literature | About e-oligos | Contact Us |   Search
July 18, 2019 e-oligos(tm) is a brand of Gene Link. e-oligos(tm) and the binary helix(tm) All graphic art is a trade mark of Gene Link, Inc. Copyright 2019
    Terms and Conditions    Licenses    Privacy Policy