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Oligo Modifications List | Oligo Modifications Reference Category
Modification : Fam dT
Reference Catalog Number 26-6422
Category Fluorescent Dyes
Modification Code Fam-dT
5 Prime Y
3 Prime Y
Internal Y
Molecular Weight (mw) 815.71
Extinction Coeficient (ec) 38.8
Technical Info (pdf) PS26-6422.pdf
Abvsorbance MAX 492
Emission MAX 517
Catalog NoScalePrice
26-6422-0550 nmol$295.00
26-6422-02200 nmol$295.00
26-6422-011 umol$390.00
26-6422-032 umol$450.00
26-6422-1010 umol$3,120.00
26-6422-1515 umol$4,500.00

Discounts are available for Fam dT!
Modification* Discount Price Structure
1 site/order List price
2 sites/order 10% discount
3 sites/order 20% discount
4 sites/order 30% discount
5-9 sites/order 50% discount
10+ sites/order 60% discount
*Exceptions apply

Related Modifications
Fam-5' (6-FAM)
Fam-NHS (6-Fam NHS)
Fam-TEG Azide

Fluorescein-dT is a deoxythymidine nucleoside derivitized with 6-FAM (6-carboxyfluorescein) through a spacer arm. 6-FAM is the most commonly used fluorescent dye for labeling oligonucleotides; Fluorescein-dT is used to internally label an oligonucleotide at a dT position. Fluorescein-dT has an absorbance maximum of 492 nm and an emission maximum of 517 nm. Fluorescein-dT can be used to internally label a Fluorescence Resonance Energy Transfer (FRET) DNA oligonucleotide probe with a fluorophore. Such a labeling strategy is pertinent in cases where the distance between the quencher and fluorophore needs optimization for efficient quenching. For such probes, fluorescein is most commonly paired with the dark quencher BHQ-1, as the two have excellent spectral overlap.

Fluorescein-dT also can be used to label DNA oligos for use as hybridization probes in a variety of in vivo and in vitro research or diagnostic applications, as well as for structure-function studies of DNA, RNA, and protein-oligonucleotide complexes. Oligos internally labeled with fluorescein-dT also can be used as PCR and DNA sequencing primers to generate fluorescently-labeled PCR, sequencing or genetic analysis (AFLP or microsatellite) products.


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