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Oligo Modifications List | Oligo Modifications Reference Category
Modification : Dabcyl-5
Reference Catalog Number 26-6704
Category Quenchers
Modification Code Dab
5 Prime Y
3 Prime Y
Internal N
Molecular Weight (mw) 462.44
Extinction Coeficient (ec) 11.1
Technical Info (pdf) PS26-6704.pdf
Catalog NoScalePrice
26-6704-0550 nmol$247.50
26-6704-02200 nmol$247.50
26-6704-011 umol$321.75
26-6704-032 umol$482.62
26-6704-1010 umol$3,217.50
26-6704-1515 umol$2,574.00

Discounts are available for Dabcyl-5!
Modification* Discount Price Structure
1 site/order List price
2 sites/order 10% discount
3 sites/order 20% discount
4 sites/order 30% discount
5-9 sites/order 50% discount
10+ sites/order 60% discount
*Exceptions apply

Dabcyl-5’ is classified as a dark quencher (a non-fluorescent chromophore), and is typically used to label a Fluorescence Resonance Energy Transfer (FRET) DNA oligonucleotide probe with a quencher moiety at the 5’-end. TaqMan or Molecular Beacon probes labeled with Dabcyl at the 5’-end usually would be labeled with an appropriate fluorescent dye at the 3’-end; other kinds of FRET probes might have the fluorescent dye at an internal position. 5’-end labeling such probes with Dabcyl is unusual. The primary reason for using this inverse-labeling strategy is to take advantage of Dabcyl’s very high hydrophobicity (which is higher than that of a fluorescent dye) to improve subsequent purification of the probe by HPLC or RPC. For example, a molecular beacon with 5’-Dabcyl and 3’-6-FAM is easier to purify (and will subsequently be purer) than a probe with the more common, opposite set-up.


Quencher Spectral Data

Quencher

Absorption Max, nm

Quenching Range, nm

Dabcyl 453 380-530
BHQ1 534 480-580
BHQ2 579 550-650
BHQ3 672 620-730
BBQ-650 650 550-750
Click here for complete list of quenchers
**Black Hole Quencher License Agreement
"Black Hole Quencher", "BHQ-1", "BHQ-2" and "BHQ-3" are registered trademarks of Biosearch Technologies, Inc., Novato, CA. The BHQ technology is licensed and sold under agreement with Biosearch and these products are sold exclusively for R&D use by the purchaser. They may not be used for clinical or diagnostic purposes and they may not be resold, distributed or re-packaged.

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