Biotin-NHS is an N-hydroxysuccinimide ester (NHS ester) of biotin. Biotin-NHS can be used to internally label an oligonucleotide with biotin at a non-T position (that is, at a G, C, or A position). To accomplish this, amino dG-C6/dC-C6/dA-C6 is first incorporated into the oligonucleotide, thereby placing an active primary amino group at the desired position. Biotin-NHS is then conjugated to the amino group in a separate reaction to form the final biotin-labeled product.
Biotin-NHS can also be used to biotinylate a large amount of oligonucleotide aminated at the 5’- or 3’-end, in aqueous solution and at relatively low cost (1).
References
1. Bengtstron, M., Jungell-nortamo, A., Syvanen, A-C. Biotinylation of Oligonucleotides Using a Water Soluble Biotin Ester.Nucleos. Nucleot. Nucl. (1990), 9: 123-127.
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