Carboxytetramethylrhodamine (TAMRA) is a fluorescent dye that is a derivative of rhodamine, and is used to label oligonucleotides at the 5’- or 3’-ends, or internally. TAMRA has an absorbance maximum of 565 nm and an emission maximum of 580 nm. TAMRA-modified oligonucleotides play a particularly important role in both fluorescence resonance energy transfer (FRET) and real-time PCR applications. |