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Oligo Modifications List | Oligo Modifications Reference Category
Modification : 3'-rG (2'-5' linked)
Reference Catalog Number 26-6912
Category Minor Bases
Modification Code 3'-rG(2'-5')
5 Prime Y
3 Prime Y
Internal Y
Molecular Weight (mw) 345.21
Extinction Coeficient (ec) 11.5
Technical Info (pdf) PS26-6912.pdf
Catalog NoScalePrice
26-6912-0550 nmol$88.40
26-6912-02200 nmol$88.40
26-6912-011 umol$114.92
26-6912-032 umol$172.38
26-6912-1010 umol$919.36
26-6912-1515 umol$1,149.20
26-6912-1515 umol$1,149.20

Discounts are available for 3'-rG (2'-5' linked) !
Modification* Discount Price Structure
1 site/order List price
2 sites/order 10% discount
3 sites/order 20% discount
4 sites/order 30% discount
5-9 sites/order 50% discount
10+ sites/order 60% discount
*Exceptions apply

Related Modifications
3'-dT (2'-5' linked)
3'-rC (2'-5' linked)
3'-rU (2'-5' linked)
3'-dG (2'-5' linked)
3'-rA (2'-5' linked)
3'-dC (2'-5' linked)
3'-dA (2'-5' linked)

3'-riboguanosine (3’-rG)–(2'-5' linked), and the other three 3’-ribonucleotide (2’,5’-linked) modifications are used to substitute 2’-5’ phosphodiester linkages for the usual 3’-5’ phosphodiester linkages at some or all positions of an oligonucleotide. Oligonucleotides containing all, or primarily, 2’,5’-phosphodiester linkages selectively bind to complementary single-stranded 3’,5’-RNA over comparable 3’,5’-DNA (1). Presumably this selectively is a consequence of the 2’,5’-linkages destabilizing duplexes formed with 3’,5’-DNA more than those formed with 3’,5’-RNA, leading to 2’5’-RNA:3’,5’-DNA duplexes having much lower Tm than the corresponding 2’5’-RNA:3’,5’-RNA duplexes. This property means that RNA oligos containing such linkages could be useful in anti-sense applications, as ssRNA-specific probes, or as ligands for affinity purification of cellular RNA.

1. Giannaris, P.A.; Damha, M.J. Oligoribonucleotides containing 2’,5’-phosphodiester linkages exhibit binding selectivity for 3’,5’-RNA over 3’,5’-ssDNA. Nucleic Acids Res (1993), 21: 4742-4749.

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