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Oligo Modifications List | Oligo Modifications Reference Category
Modification : ddT-5'
Reference Catalog Number 26-6334
Category End Blockers
Modification Code ddT-5'
5 Prime Y
3 Prime N
Internal N
Molecular Weight (mw) 288.19
Extinction Coeficient (ec) 8.7
Technical Info (pdf) PS26-6334.pdf
Catalog NoScalePrice
26-6334-0550 nmol$195.00
26-6334-02200 nmol$195.00
26-6334-011 umol$292.00
26-6334-032 umol$438.00
26-6334-1010 umol$2,336.00
26-6334-1515 umol$2,920.00

Discounts are available for ddT-5'!
Modification* Discount Price Structure
1 site/order List price
2 sites/order 10% discount
3 sites/order 20% discount
4 sites/order 30% discount
5-9 sites/order 50% discount
10+ sites/order 60% discount
*Exceptions apply

Related Modifications
ddA-5'
ddG-5'
ddT-5'
ddC-3'
ddC-5'

Dideoxythymidine (ddT) is a dideoxyribonucleoside that can only be used to block the 5' end. Use ddC-3' or Spacer C3 to block the 3' end from polymerase extension. ddT is a synthetic analog of deoxythymidine, in ddT, both the 2’- and 3’-positions of the ribose have a hydrogen (-H) group substituted for the –OH group, whereas in dT, only the 2’-position is so substituted. ddT is added to the 5’-end of an oligo via 5’-to-5’ synthesis, using a 2’,3’-ddT, 5’-phosphoramidite. Purification must be by PAGE purification, since such an oligo will not have a trityl group that is required for RPC purification.

ddC-3' should be used as a 3’-end blocking moiety (see ddC technical sheet).

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