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Oligo Modifications List | Oligo Modifications Reference Category
Modification : FAM-TEG Azide
Reference Catalog Number 26-6722
Category Click Chemistry
Modification Code FAM-TEG-N3
5 Prime Y
3 Prime Y
Internal Y
Molecular Weight (mw) 576.55
Technical Info (pdf) PS26-6722.pdf
Catalog NoScalePrice
26-6722-0550 nmol$540.00
26-6722-02200 nmol$540.00
26-6722-011 umol$702.00
26-6722-032 umol$1,053.00
26-6722-1010 umol$5,616.00
26-6722-1515 umol$7,020.00

6-FAM (6-carboxyfluorescein)-TEG Azide is a 6-FAM fluorescent dye attached to a 15-atom mixed polarity triethylene glycol spacer with an azide group at the end of the spacer. 6-FAM is the most commonly used fluorescent dye for labeling oligonucleotides, and is reactive and water-soluble, with an absorbance maximum of 492 nm and an emission maximum of 517 nm. The presence of the azide allows the user to use “Click Chemistry” (a [3+2] cycloaddition reaction between alkynes and azides, using copper (I) iodide as a catalyst) to conjugate the 6-FAM-TEG Azide to a terminal alkyne-modified oligo with extremely high regioselectivity and efficiency (1,2). Preparation of the alkyne-modified oligo can be achieved using the 5’-Hexynyl modifier (see its respective tech sheet for details). The spacer acts to minimize steric hindrance between the biotin moiety and the oligo.

References
1. Huisgen, R. Angew. Chem. Int. Ed. (1963), 2: 565-568.
2. Rostovtsev, V.V., Green, L.G., Fokin, V.V., Sharpless, K.B. A Stepwise Huisgen Cycloaddition Process: Copper(I)-Catalyzed Regioselective Ligation of Azides and Terminal Alkynes. Angew. Chem. Int. Ed. (2002), 41: 2596-2599.
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