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Oligo Modifications List | Oligo Modifications Reference Category
Modification : Atto Rho101
Reference Catalog Number 26-6970
Category Fluorescent Dyes
Modification Code AttoRho101
5 Prime Y
3 Prime Y
Internal Y
Molecular Weight (mw) 892
Technical Info (pdf) PS26-6970.pdf
Abvsorbance MAX 586
Emission MAX 610
Catalog NoScalePrice
26-6970-0550 nmol$650.00
26-6970-02200 nmol$650.00
26-6970-011 umol$845.00
26-6970-032 umol$2,550.00
26-6970-1010 umol$6,760.00
26-6970-1515 umol$8,450.00

This modification is a post synthesis conjugation to a primary amino group thus an additional modification with an amino group is required. A C3, C6 or C12 amino group can be placed at the 5' or for the 3' end a C3 or C7 amino and for internal positions an amino modified base is used, e.g Amino dT C6.

YIELD
NHS based modifications are post synthesis conjugation performed using a primary amino group. The yield is lower as compared to direct automated coupling of modifications that are available as amidites. Approximate yield for various scales are given below.

~2 nmol final yield for 50 nmol scale synthesis.
~5 nmol final yield for 200 nmol scale synthesis.
~16 nmol final yield for 1 umol scale synthesis

ATTO Rho101 is a derivative of the well-known dye Rhodamine 101. Characteristic features of the label are strong absorption, extraordinarily high fluorescence quantum yield, and high thermal and photo-stability. The dye is moderately hydrophilic. ATTO Rho101 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1.

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