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Oligo Modifications List | Oligo Modifications Reference Category
Modification : NTA (Nitrilotriacetate) Oligo Conjugate
Reference Catalog Number 26-6444
Category Affinity Ligands
Modification Code NTA
5 Prime Y
3 Prime Y
Internal Y
Molecular Weight (mw) 489.38
Technical Info (pdf) PS26-6444.pdf
Catalog NoScalePrice
26-6444-0550 nmol$350.00
26-6444-02200 nmol$350.00
26-6444-011 umol$625.00
26-6444-032 umol$937.00
26-6444-1010 umol$4,400.00
26-6444-1515 umol$5,280.00
Related Modifications
Thiol C6 dT
Thiol SS Dipod (DTSPA)
Thiol SS Dipod (DTPA)
Thiol SS-C3
Thiol SS-C6

YIELD
NTA modified oligos.

~ 2 nmol final yield for 50 nmol scale synthesis.
~ 6 nmol final yield for 200 nmol scale synthesis.
~ 20 nmol final yield for 1 umol scale synthesis.

NTA-Oligo modified with nitrilotriacetate (NTA)is a post synthesis conjugation to thiol. The thiol group can be placed at the 5' and 3' and for internal positions DTSPA can be used. The price listed is for NTA modification and does not include thiol modification, oligo synthesis and purification charges

NTA-Oligo modified with nitrilotriacetate (NTA), which has high affinity to a His-tag on recombinant protein via the complexation of Ni2+.
It is a novel method to prepare a DNA-protein conjugate using histidine-tag (His-tag) chemistry.

References


1. J. Shimada, T. Maruyama, T. Hosogi, J. Tominaga, N. Kamiya, M. Goto,. Conjugation of DNA with protein using His-tag chemistry and its application to the aptamer-based detection system, Biotechnol. Lett. 30 (2008) 2001–2006.
2.J. Shimada et al DNA–enzyme conjugate that can detect thrombin. Anal. Biochem. 414 (2011) 103–108.
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