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Oligo Modifications List | Oligo Modifications Reference Category
Modification : DBCO-dT
Reference Catalog Number 26-6927
Category Click Chemistry
Modification Code DBCO-dT
5 Prime Y
3 Prime Y
Internal Y
Molecular Weight (mw) 773.77
Technical Info (pdf) PS26-6927.pdf
Catalog NoScalePrice
26-6927-0550 nmol$485.00
26-6927-02200 nmol$485.00
26-6927-011 umol$610.00
26-6927-032 umol$915.00
26-6927-1010 umol$4,880.00
26-6927-1515 umol$6,100.00

Discounts are available for DBCO-dT!
Modification* Discount Price Structure
1 site/order List price
2 sites/order 10% discount
3 sites/order 20% discount
4 sites/order 30% discount
5-9 sites/order 50% discount
10+ sites/order 60% discount
*Exceptions apply

Related Modifications
Azide-C4 NHS (butyrate)
Azide C3 3'
Azide-C2 NHS
BCN-1 (bi cyclooctyne)

DBCO-TEG and DBCO-dT direct conjugation modifications have been discontinued due to instability and low overall performance. We suggest to use the alternates that are post synthesis conjugation using DBCO-NHS for amino conjugation or DBCO-Maleimide for thiol conjugation.

Click here for a list of post synthesis DBCO Modifications

DBCO conjugation chemistry is based on the reaction of a dibenzylcyclooctyne (DBCO) linker with an azide linker to form a stable triazole. The dibenzocyclooctyne group (DBCO) allows Copper-free Click Chemistry to be done with live cells, whole organisms, and non-living samples. DBCO groups will preferentially and spontaneously label molecules containing azide groups (– N3). Within physiological temperature and pH ranges, the DBCO group does not react with amines or hydroxyls, which are naturally present in many biomolecules. Reaction of the DBCO group with the azide group is significantly faster than with the sulfhydryl group (–SH, thiol).

Cyclooctyne-based modifications offers the ease of copper-free click reagents. These are simple to use and has excellent click performance in 17 hours or less at room temperature. Gene Link offers 5'-DBCO-TEG for preparing oligos with 5’-DBCO and a 15 tom triethylene glycol spacer arm, DBCO-dT for inserting a DBCO group at any position within the oligonucleotide and DBCO-sulfo-NHS Ester is also offered for post-synthesis conjugation reactions. DBCO-modified oligos may be conjugated with azides in organic solvents, such as DMSO, or aqeous buffers. Depending on the azide used, the reaction will go to completion in 4-17 hours at room temperature.

Glen Report 27.1: Technical Brief - DBCO-dT - An Unusual Case of Iodine Sensitivity


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