DBCO-TEG and DBCO-dT direct conjugation modifications have been discontinued due to instability and low overall performance. We suggest to use the alternates that are post synthesis conjugation using DBCO-NHS for amino conjugation or DBCO-Maleimide for thiol conjugation.
Click here for a list of post synthesis DBCO Modifications
Cyclooctyne-based modifications offers the ease of copper-free click reagents. These are simple to use and has excellenet click performance in 17 hours or less at room temperature. Gene Link offers 5'-DBCO-TEG for preparing oligos with 5’-DBCO and a 15 tom triethylene glycol spacer arm, DBCO-dT for inserting a DBCO group at any position within the oligonucleotide and DBCO-sulfo-NHS Ester is also offered for post-synthesis conjugation reactions. DBCO-modified oligos may be conjugated with azides in organic solvents, such as DMSO, or aqeous buffers. Depending on the azide used, the reaction will go to completion in 4-17 hours at room temperature.
Glen Report 27.1: Technical Brief - DBCO-dT - An Unusual Case of Iodine Sensitivity