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Oligo Modifications List | Oligo Modifications Reference Category
Modification : TET
Reference Catalog Number 26-6433
Category Fluorescent Dyes
Modification Code TET
5 Prime Y
3 Prime Y
Internal N
Molecular Weight (mw) 675.24
Extinction Coeficient (ec) 16.255
Technical Info (pdf) PS26-6433.pdf
Abvsorbance MAX 522
Emission MAX 538
Catalog NoScalePrice
26-6433-0550 nmol$90.00
26-6433-02200 nmol$90.00
26-6433-011 umol$135.00
26-6433-032 umol$202.50
26-6433-1010 umol$1,080.00
26-6433-1515 umol$1,350.00

Tetrachloro fluorescein (TET) is tetra-chloro derivative of fluorescein that is used to fluorescently label oligonucleotides. TET has an absorbance maximum of 522 nm and an emission maximum of 538 nm. TET plays a role in real-time PCR applications, being used as a reporter moiety in TaqMan probes (1), Scorpion primers (2) and Molecular Beacons (3). For such probes, TET is most commonly paired with the dark quencher BHQ-1, as the two have excellent spectral overlap.

TET can be used to label DNA oligos for use as hybridization probes in a variety of in vivo and in vitro research or diagnostic applications, as well as for structure-function studies of DNA, RNA, and protein-oligonucleotide complexes. Oligos labeled with TET at the 5’-end can be used as PCR and DNA sequencing primers to generate fluorescently-labeled PCR, sequencing or genetic analysis (AFLP or microsatellite) products.

References
1. Livak, K.J., Flood, S.J.A., Marmaro, J., Giusti, W., Deetz, K. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization.PCR Methods Appl. (1995), 4: 1-6.
2. Thelwell, N., Millington, S., Solinas, A., Booth, J., Brown, T. Mode of action and application of Scorpion primers to mutation detection. Nucleic Acids Res. (2000), 28: 3752-3761.
3. Tyagi, S., Kramer, F.R. Molecular beacons: probes that fluoresce upon hybridization. Nat. Biotechnol. (1996), 14: 303-308.
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