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Oligo Modifications List | Oligo Modifications Reference Category
Modification : Marina Blue
Reference Catalog Number 26-6687
Category Fluorescent Dyes
Modification Code MrBl NHS
5 Prime Y
3 Prime Y
Internal Y
Molecular Weight (mw) 284.22
Technical Info (pdf) PS26-6687.pdf
Abvsorbance MAX 365
Emission MAX 460
Catalog NoScalePrice
26-6687-0550 nmol$350.00
26-6687-02200 nmol$350.00
26-6687-011 umol$455.00
26-6687-032 umol$682.00
26-6687-1010 umol$3,640.00
26-6687-1515 umol$4,550.00

Marina Blue is a proprietary dye manufactured by Life Tech/ThermoFisher, it is available as a fluorescent dye that can be conjugated to an oligo, either RNA or DNA. This is a post synthesis conjugation to a primary amino group. The amino group can be placed at the 5' and 3' and for internal positions an amino modified base is used, e.g Amino dT C6

The amine-reactive Marina Blue® succinimidyl ester can be used to create blue-fluorescent bioconjugates. Based on the 6,8-difluoro-7-hydroxycoumarin fluorophore, Marina Blue® dye exhibits bright blue fluorescence emission near 460 nm and is optimally excited by the intense 365 nm spectra line of the mercury-arc lamp. Because the pKa values of Marina Blue® derivatives are 2-3 log units lower than those of the corresponding 7-hydroxycoumarin conjugates, Marina Blue® conjugates are strongly fluorescent even at neutral pH.

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