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Oligo Modifications List | Oligo Modifications Reference Category
Modification : Tamra-dT
Reference Catalog Number 26-6449
Category Fluorescent Dyes
Modification Code Tm-dT
5 Prime Y
3 Prime Y
Internal Y
Molecular Weight (mw) 870.85
Extinction Coeficient (ec) 40.38
Technical Info (pdf) PS26-6449.pdf
Abvsorbance MAX 565
Emission MAX 580
Catalog NoScalePrice
26-6449-0550 nmol$350.00
26-6449-02200 nmol$350.00
26-6449-011 umol$450.00
26-6449-032 umol$675.00
26-6449-1010 umol$3,600.00
26-6449-1515 umol$4,500.00
Related Modifications
Tamra-3'
Tamra-5'
Tamra-dT
Tamra NHS

Carboxytetramethylrhodamine-deoxythymidien (TAMRA-dT) is a deoxythymidine nucleoside derivitized with TAMRA through a spacer arm. TAMRA-dT is used to internally label an oligonucleotide at a dT position. TAMRA-dT has an absorbance maximum of 565 nm and an emission maximum of 580 nm. TAMRA-dT can be used to internally label a Fluorescence Resonance Energy Transfer (FRET) DNA oligonucleotide probe with a quencher moiety. Such a labeling strategy is pertinent in cases where the distance between the quencher and fluorophore needs optimization for efficient quenching. For such probes, 6-FAM is most commonly used as the reporter moiety as the two dyes have excellent spectral overlap.

TAMRA-dT also can be used to label DNA oligos for use as hybridization probes in a variety of in vivo and in vitro research or diagnostic applications, as well as for structure-function studies of DNA, RNA, and protein-oligonucleotide complexes. Oligos internally labeled with TAMRA-dT also can be used as PCR and DNA sequencing primers to generate fluorescently-labeled PCR, sequencing or genetic analysis (AFLP or microsatellite) products. For further details concerning the TAMRA dye, please see the technical sheet for it.

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