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Oligo Modifications List | Oligo Modifications Reference Category
Modification : Amino Allyl
Reference Catalog Number 26-6731
Category Conjugation Chemistry & End Modifiers
Modification Code AmAllyl
5 Prime Y
3 Prime Y
Internal Y
Molecular Weight (mw) 101.14
Technical Info (pdf) 26-6731.pdf
Catalog NoScalePrice
26-6731-0550 nmol$210.00
26-6731-02200 nmol$210.00
26-6731-011 umol$256.00
26-6731-032 umol$310.00
26-6731-1010 umol$1,550.00
26-6731-1515 umol$1,755.00

Discounts are available for Amino Allyl!
Modification* Discount Price Structure
1 site/order List price
2 sites/order 10% discount
3 sites/order 20% discount
4 sites/order 30% discount
5-9 sites/order 50% discount
10+ sites/order 60% discount
*Exceptions apply

Related Modifications
Amino C6
Amino dA C6
Amino Serinol
Amino Allyl rU
Amino dT C6
Amino Allyl dU
Amino dG C6
Amino C3
Amino C12
Amino C6 U
Amino C6 Universal
Amino C7
Amino dC C6

Amino Allyl can be used to incorporate an active primary amino group onto the 5'-end of an oligonucleotide. This can then be conjugated to a NHS Activated ligand. The amino group then becomes internal to the 5' end ligand. The amino group is separated from the 5'-end nucleotide base by a 4-carbon spacer arm to reduce steric interaction between the amino group and the oligo.
The presence of the primary amino group allows the user to label the oligo with a variety of different ligands for affinity, reporter or protein moieties (as NHS esters or isothiocyanates), depending on the application. Examples include biotin, digoxigenin, and fluorescent dyes or quenchers, magnetic beads and enzymes (for example, alkaline phosphatase).


The primary amine labelled oligos can also be conjugated to carboxyl functional groups usually for solid supports applications using EDC mediated reaction as shown in the figure below.


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