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Oligo Modifications List | Oligo Modifications Reference Category
Modification : Tet-NHS (Tetrachloro-Fluorescein)
Reference Catalog Number 26-6594
Category Fluorescent Dyes
Modification Code Tet-N
5 Prime Y
3 Prime Y
Internal Y
Molecular Weight (mw) 675.24
Extinction Coeficient (ec) 16.255
Technical Info (pdf) PS26-6594.pdf
Abvsorbance MAX 522
Emission MAX 538
Absorbance EC 16.255
Catalog NoScalePrice
26-6594-0550 nmol$195.00
26-6594-02200 nmol$195.00
26-6594-011 umol$285.00
26-6594-032 umol$427.00
Related Modifications
HEX-NHS (Hexachloro-Fluorescein)
Fam-3' (6-FAM)
Fam-5' (6-FAM)

Tetrachloro fluorescein (TET) is tetra-chloro derivative of fluorescein that is used to fluorescently label oligonucleotides. TET has an absorbance maximum of 522 nm and an emission maximum of 538 nm. TET plays a role in real-time PCR applications, being used as a reporter moiety in TaqMan probes (1), Scorpion primers (2) and Molecular Beacons (3). For such probes, TET is most commonly paired with the dark quencher BHQ-1, as the two have excellent spectral overlap.

TET can be used to label DNA oligos for use as hybridization probes in a variety of in vivo and in vitro research or diagnostic applications, as well as for structure-function studies of DNA, RNA, and protein-oligonucleotide complexes. Oligos labeled with TET at the 5' end can be used as PCR and DNA sequencing primers to generate fluorescently-labeled PCR, sequencing or genetic analysis (AFLP or microsatellite) products.

1. Livak, K.J., Flood, S.J.A., Marmaro, J., Giusti, W., Deetz, K. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization.PCR Methods Appl. (1995), 4: 1-6.
2. Thelwell, N., Millington, S., Solinas, A., Booth, J., Brown, T. Mode of action and application of Scorpion primers to mutation detection. Nucleic Acids Res. (2000), 28: 3752-3761.
3. Tyagi, S., Kramer, F.R. Molecular beacons: probes that fluoresce upon hybridization. Nat. Biotechnol. (1996), 14: 303-308.

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