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Oligo Modifications List | Oligo Modifications Reference Category
Modification : Acridine
Reference Catalog Number 26-6694
Category Intercalators
Modification Code Acryd
5 Prime Y
3 Prime Y
Internal N
Molecular Weight (mw) 450.86
Extinction Coeficient (ec) 39.5
Technical Info (pdf) PS26-6694.pdf
Catalog NoScalePrice
26-6694-0550 nmol$324.50
26-6694-02200 nmol$324.50
26-6694-011 umol$421.85
26-6694-032 umol$632.77
26-6694-1010 umol$3,374.80
26-6694-1515 umol$4,218.50

Discounts are available for Acridine !
Modification* Discount Price Structure
1 site/order List price
2 sites/order 10% discount
3 sites/order 20% discount
4 sites/order 30% discount
5-9 sites/order 50% discount
10+ sites/order 60% discount
*Exceptions apply

Acridine is classified as a DNA intercalating agent. Labeling of DNA oligos with acridine allows them to rapidly and stably intercalate into a target dsDNA molecule, adding increased stability to the double helix (1). In addition, incorporation of acridine to the 3'-end of an oligo confers a high level of exonuclease resistance to that end (2). Thus, oligos labeled with acridine may be useful in applications involving oligo hybrids requiring higher levels of stability.

Acridine-labeled oligos containing a polypyrimidine sequence possess the ability to form triplex helices that are highly stable, and, due to their increased hydrophobicity, can pass through membranes more easily than normal oligos. Such oligos were used as anti-sense reagents to suppress c-myc protooncogene expression and control tumor growth in mice (3), and as a transcriptional repressor to the IL-2 Receptor (4).

Since acridine is a fluorescent dye, it can also be use to make dye-labeled oligos. An interesting such application is the use of acridine-labeled primers to study non-enzymatic-template-directed RNA synthesis to provide experimental support for theories concerning possible replication of genetic information by early life forms on Earth (5).

References
1. Fukui, K., Tanaka, K. The Acridine Ring Selectively Intercalated into a DNA Helix at Various Types of Abasic Sites: Double Strand Formation and Photophysical Properties. Nucleic Acids Res. (1996), 24: 3962-3967.
2. Gamper, H.B., Reed, M.W., Cox, T., Virosco, J.S., Adams, A.D., Gall, A.A., Scholler, J.K., Meyer, R.B. Facile preparation of nuclease resistant 3� modified oligodeoxynucleotides. Nucleic Acids Res. (1993), 21: 145-150.
3. Stewart, D.A., Xu, Xiaohou, Thomas, S.D., Miller, D.M. Acridine-modified, clamp-forming antisense oligonucleotides synergize with cisplatin to inhibit c-Myc expression and B16-F0 tumor progression.Nucleic Acids Res. (2002), 30: 2565-2574.
4. Grigoriev, M., Praseuth, D., Robin, P., Hemar, A., Saison-Behmoaras, T., Dautry-Varsat, A., Thuong, N.T., Helene, C., Harel-Bellan, A. A Triple-Helix-forming Oligonucleotide-Intercalator Conjugate Acts as Transcriptional Repressor via Inhibition of NFkB Binding to Interleukin-2 Receptor alpha-Regulatory Sequence. J. Biol. Chem. (1992), 267: 3389-3395.
5. Kurz, M., Gobel, K., Hartel, C., Gobel, M.W. Acridine-labeled primers as tools for the study of nonenzymatic RNA oligomerization. Helv. Chim. Acta (1998), 81: 1156-1180.
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