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Oligo Modifications List | Oligo Modifications Reference Category
Modification : Cy7 NHS
Reference Catalog Number 26-6474
Category Fluorescent Dyes
Modification Code Cy7-NHS
5 Prime Y
3 Prime Y
Internal Y
Molecular Weight (mw) 682
Extinction Coeficient (ec) 12.5
Technical Info (pdf) PS26-6474.pdf
Abvsorbance MAX 747
Emission MAX 776
Catalog NoScalePrice
26-6474-0550 nmol$325.20
26-6474-02200 nmol$325.20
26-6474-011 umol$422.76
26-6474-032 umol$634.14
26-6474-1010 umol$3,382.08
26-6474-1515 umol$4,227.60
Related Modifications
Cy5 disulfo NHS
Cy3
Cy2 NHS
Cy5.5
Cy3 NHS
Cy3.5
Cy3B
Cy5
Cy5 NHS

Cy7 modification is a post synthesis conjugation to a primary amino group thus an additional modification with an amino group is required. A C6 or C12 amino group can be placed at the 5' or for the 3' end a C3 or C7 amino and for internal positions an amino modified base is used, e.g Amino dT C6.

Cyanine 7(Cy7) NHS ester is a fluorescent dye that belongs to the Cyanine family of synthetic polymethine dyes. Cy7 is reactive, water-soluble, and has an absorbance maximum of 747 nm and an emission maximum of 776 nm, which is in the near IR. It is available as an NHS ester, and is used to fluorescently label oligonucleotides at either the 5’- or 3’-end, or internally. Because it is a near IR dye, Cy7 has very little background fluorescence associated with it (1). It is thus an excellent choice for labeling oligo probes slated for in vivo applications, because the minimal scattering and absorption of near-IR photons by cellular tissue ensures higher S/N ratio, and better sensitivity. For example, Fluorescent Resonance Energy Transfer (FRET) oligonucleotide duplexes using Cy5.5 as the donor on one strand and Cy7 as the acceptor on the complementary strand have been used to detect and characterize transcription factor NF-kappaB p50 protein binding to DNA (2)

Caution: Cy7 is intensely colored and very reactive. Care should be exercised when handling the vial containg the C7-labeled oligo to avoid staining clothing, skin, and other items. Also, because Cy7 is in the form of an NHS ester, the oligo first must be synthesized with an Amino C6 Linker (for the ends) or the Amino C6 version of the base phosphoramidite (for internal labeling). The Cy7-NHS ester is then manually attached to the oligo through the amino group in a separate reaction post-synthesis.

References
1. Benson, R.C., Kues, H.A. Absorption and Fluorescence Properties of Cyanine Dyes.J. Chem. Eng. Data (1977), 22: 379-383.
2. Zhang, S., Metelev, V., Tabatadze, D., Zamecnik, P.C., Bogdanov, A. Fluorescence resonance energy transfer in near-infrared fluorescent oligonucleotide probes for detecting protein-DNA interactions. Proc. Nat. Acad. Sci. USA. (2008), 105: 4156-4161.
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