Black Hole Quencher-1 deoxythymidine (BHQ1-dT) is classified as a dark quencher (a non-fluorescent chromophore) nucleotide base, and is typically used to internally label a Fluorescence Resonance Energy Transfer (FRET) DNA oligonucleotide probe with a quencher moiety. Such a labeling strategy is pertinent in cases where the distance between the quencher and fluorophore needs optimization for efficient quenching. Other basic technical information about BHQ-1 is found in the BHQ-1 technical sheet.
Quencher Spectral Data
|
Quencher
|
Absorption Max, nm
|
Quenching Range, nm
|
Dabcyl |
453 |
380-530 |
BHQ-0 |
495 |
430-520 |
BHQ1 |
534 |
480-580 |
BHQ2 |
579 |
550-650 |
BHQ3 |
672 |
620-730 |
BBQ-650 |
650 |
550-750 |
Click here for complete list of quenchers
|
**Black Hole Quencher License Agreement
Black Hole Quencher License Agreement.
""Black Hole Quencher®, BHQ®, CAL Fluor® and Quasar® are registered trademarks of
Biosearch Technologies, Inc., Petaluma, California.
The BHQ, CAL Fluor and Quasar dye technologies are protected by U.S. and world-wide patents either issued or in application. Compounds
incorporating these dyes are made and sold under agreement with Biosearch Technologies, Inc. for end-user's non-commercial research and development use only.
Their use in commercial applications is encouraged but requires a separate Commercial Use License granted by Biosearch Technologies, Inc."
|
|