Black Hole Quencher-1 deoxythymidine (BHQ1-dT) is classified as a dark quencher (a non-fluorescent chromophore) nucleotide base, and is typically used to internally label a Fluorescence Resonance Energy Transfer (FRET) DNA oligonucleotide probe with a quencher moiety. Such a labeling strategy is pertinent in cases where the distance between the quencher and fluorophore needs optimization for efficient quenching. Other basic technical information about BHQ-1 is found in the BHQ-1 technical sheet.
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Quencher Spectral Data
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Quencher
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Absorption Max, nm
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Quenching Range, nm
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| Dabcyl |
453 |
380-530 |
| BHQ-0 |
495 |
430-520 |
| BHQ1 |
534 |
480-580 |
| BHQ2 |
579 |
550-650 |
| BHQ3 |
672 |
620-730 |
| BBQ-650 |
650 |
550-750 |
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Click here for complete list of quenchers
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**Black Hole Quencher License Agreement
Black Hole Quencher License Agreement.
""Black Hole Quencher®, BHQ®, CAL Fluor® and Quasar® are registered trademarks of
Biosearch Technologies, Inc., Petaluma, California.
The BHQ, CAL Fluor and Quasar dye technologies are protected by U.S. and world-wide patents either issued or in application. Compounds
incorporating these dyes are made and sold under agreement with Biosearch Technologies, Inc. for end-user's non-commercial research and development use only.
Their use in commercial applications is encouraged but requires a separate Commercial Use License granted by Biosearch Technologies, Inc."
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