Spacer C12 is a 12-carbon spacer that is used to incorporate a long spacer arm into an oligonucleotide. Spacer C12 can be incorporated in consecutive additions if a longer spacer is required. Spacer C12 is frequently used for solid-phase immobilization of DNA probes or aptamers for microarray applications (1,2), but can be used for any oligonucleotide-based application requiring a long spacer arm.
1. Reese, M.O., van Dam, R.M., Scherer, A., Quake, S.R. Microfabricated Fountain Pens for High-Density DNA Arrays. Genome Res. (2003), 13: 2348-2352.
2. Lao, Y-H., Peck, K., Chen, L-C. Enhancement of Aptamer Microarray Sensitivity through Spacer Optimization and Avidity Effect. Anal. Chem. (2006), 81: 1747-1754.