Molecular Beacons Technical Information Molecular Beacons FAQ's
TaqMan probes with Tamra at 3' end and Molecular
beacons with universal fluorescence quencher DABCYL at 3’ end. Price includes gel purification. Gene Link considers
gel purification to be the best method of purification and essential for
optimum performance of fluorescent dye labeled oligonucleotides.Customers may request Molecular Beacons without gel purification for reduced pricing.
|
Fluorophore*
|
Color
|
Absorbance
max (nm)
|
Emission
max (nm)
|
$,
200 nmol scale
|
$,
1 mmol
scale
|
|
Dabcyl
(Quencher)
|
|
453
|
|
$290
|
$350
|
|
6-FAM
(Fluorescein)
|
Green
|
494
|
525
|
$390
|
$450
|
|
TET
|
Orange
|
521
|
536
|
$390
|
$450
|
|
HEX
|
Pink
|
535
|
556
|
$390
|
$450
|
|
Cy
5
|
Violet
|
646
|
667
|
$490
|
$550
|
|
Cy
5.5
|
Blue
|
683
|
707
|
$490
|
$550
|
|
Cy
3
|
Red
|
552
|
570
|
$490
|
$550
|
|
Cy
3.5
|
Purple
|
588
|
604
|
$490
|
$550
|
|
Tetramethylrhodamine
|
Rose
|
565
|
580
|
$540
|
$600
|
Volume
and contract discount pricing available.
Alexa Dye series
|
Alexa
Fluor Dye
|
Color
|
Absorbance
max
(nm)
|
Emission
max (nm)
|
Extinction
Coefficient
|
|
Alexa
Fluor 350
|
|
346
|
442
|
19,000
|
|
Alexa
Fluor 405
|
|
401
|
421
|
34,000
|
|
Alexa
Fluor 430
|
|
433
|
541
|
16,000
|
|
Alexa
Fluor 488
|
|
495
|
519
|
71,000
|
|
Alexa
Fluor 532
|
|
532
|
553
|
81,000
|
|
Alexa
Fluor 546
|
|
556
|
573
|
104,000
|
|
Alexa
Fluor 555
|
|
555
|
565
|
150,000
|
|
Alexa
Fluor 568
|
|
578
|
603
|
91,000
|
|
Alexa
Fluor 594
|
|
590
|
617
|
73,000
|
|
Alexa
Fluor 633
|
|
632
|
647*
|
100,000
|
|
Alexa
Fluor 647
|
|
650
|
665*
|
239,000
|
|
Alexa
Fluor 660
|
|
663
|
690*
|
123,000
|
|
Alexa
Fluor 680
|
|
679
|
702*
|
184,000
|
|
Alexa
Fluor 700
|
|
702
|
723*
|
192,000
|
|
Alexa
Fluor 750
|
|
749
|
775*
|
240,000
|
Spectral characteristics of the Alexa Fluor dyes.
Extinction coefficient at l
max in cm -1m-1. *Human
vision is insensitive to light beyond~650nm, and therefore it is not
possible to view the far -red- fluorescent dyes by looking through the
eyepiece of a conventional fluorescent microscope. Source: Molecular
Probes www.probes.com
List
of Other Molecular Probes Fluorescent Dyes
|
Dye
|
Color
|
Absorption
Maximum (nm)
|
Emission
Maximum (nm)
|
|
Cascade Blue
|
Blue
|
396
|
410
|
|
Marina Blue
|
Blue
|
362
|
459
|
|
Oregon Green 500
|
Green
|
499
|
519
|
|
Oregon Green 514
|
Green
|
506
|
526
|
|
Oregon Green 488
|
Green
|
495
|
521
|
|
Oregon Green 488-X
|
Green
|
494
|
517
|
|
Pacific Blue
|
Blue
|
416
|
451
|
|
Rhodamine Green
|
Green
|
504
|
532
|
|
Rhodol Green
|
Green
|
496
|
523
|
|
Rhodamine Green-X
|
Green
|
503
|
528
|
|
Rhodamine Red-X
|
Red
|
560
|
580
|
|
Texas Red-X
|
Red
|
583
|
603
|
Email support@genelink.com or call 1-800-GENE LINK for
more technical
information.
*Disclaimer of License Statement for Molecular Beacons Products
The 5' Nuclease detection assay and other homogeneous amplification methods used in connection with the Polymerase Chain Reaction ("PCR") Process are covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd. ("Roche"). Use of these methods requires a license. No license under these patents, which include but are not limited to United States Patent Nos. 5,210,015, 5,487,972, 5,804,375, and 5,994,076 to use the 5' Nuclease Assay or any Roche patented homogeneous amplification process is conveyed expressly or by implication to the purchaser by the purchase of any Gene Link, Inc. PCR-related product. Purchasers of these products must obtain a license to use the 5' Nuclease or Homogeneous PCR process before performing PCR. Further information on purchasing licenses to practice the PCR Process may be obtained by contacting the Licensing Specialist at (510) 814-2984, Roche Molecular Systems, Inc. 1145 Atlantic Avenue, Alameda, California, 94501
*PHRI Molecular Beacon License Agreement
"This product is sold under license from the Public Health Research Institute. It may be used under PHRI Patent Rights only for the purchaser's research and development activities".
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