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II
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All e-oligos supplied oligos
are synthesized by Gene Link. These oligos are not 'factory style' mass
produced oligos. These are for demanding applications and consistent
results.
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| Synthesis
Scale,$/base* |
| |
25
nmol |
50
nmol |
200
nmol |
1
umol |
2
umol |
10
umol |
15
umol |
| Unmodified
Oligonucleotides |
$0.35 |
$0.40 |
$0.80 |
$1.80 |
$2.80 |
$18.00 |
$22.00 |
| Guaranteed Yield OD (A260 units) |
3 |
5 |
20 |
80 |
160 |
800 |
1200 |
| Yield in nmols for a 20mer |
15+ |
30+ |
80+ |
400+ |
800+ |
4000+ |
6000+ |
| Same Day Oligo Service |
$20.00 per oligo (Must order by 12 PM EST) |
| *minimum
charges for 20 mer applies |
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Long
Oligos? Gene Link will synthesize it.
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We specialize in long oligo
synthesis. Oligos up to 250mer. How many of our
competitors will synthesize long oligos? |
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I |
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Gene
Link maintains a coupling efficiency of greater than 99% |
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Quality of oligo is
determined by the yield of full length sequence. A lower coupling
efficiency synthesis oligo will yield in lower yield of full length
product. These may still perform in single template PCR reactions but
fail in more demanding low abundance template PCR. |
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| Oligo Size and Purification Recommendations |
| Length |
Page |
HPLC/RPC |
| 8-40 mer |
Yes |
Yes |
| 41-250 mer |
Yes |
No |
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All Gene Link oligos shorter than 40 mer usually do not require any further purification if the application is for PCR or sequencing.
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| Application Based Purification Recommendations |
| Application |
Purification |
| PCR and Sequencing |
Not Required |
| Cloning and Gene Construction |
Yes |
| Mutagenesis |
Yes |
| Modified Oligos |
Yes |
| Probes |
Yes |
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Compare the yield difference
at apparently good coupling efficiency of 98% and higher. |
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II |
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